Transcriptional Changes Associated with Long-Term Left Ventricle Volume Overload in Rats: Impact on Enzymes Related to Myocardial Energy Metabolism

Figure 1

(a) Heat map of expression of 22 genes associated with FAO in LV of 9-month rats. Levels of expression are illustrated from the highest (bright yellow) to the lowest (dark blue). Five animals were studied per group and their expression levels are illustrated individually. (b) Comparison between fold change results obtained from the microarray and by quantitative RT-PCR for a subset of 16 genes illustrated in (a). Results are reported in fold change compared to sham controls as the mean ± SEM ( per group for array and for qRT-PCR). Levels in sham animals were fixed to 1. (c) PPARα, PGC1α, and RXRs LV mRNA levels in 9-month AR rats are strongly downregulated. Exact values are indicated when two groups were compared. and between groups. (d) Evaluation of capillaries density in LV myocardium, expressed as the number of capillaries per field (a). Results are reported as mean ± SEM (/gr). Representative LV sections from sham-operated or AR rats showing isolectin B4-stained capillaries (brown-red) are illustrated.

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