The MicroRNA3686 Inhibits the Proliferation of Pancreas Carcinoma Cell Line by Targeting the Polo-Like Kinase 1

Figure 2

Pole-like kinase 1 (PLK1) is the regulation target of microRNA3686. (a) Luciferase based reporter assay for validating the PLK1 as the target of mir3686. The 3′UTR containing putative mir3686 targeting sequence was cloned to a luciferase report vector and cotransfected to HEK293T cell with mir3686 mimic or scramble control; the luciferase activities was evaluated by Dual-Glo Luciferase Assay System. Error bars represent standard errors of three repeated experiments. Significant differences between groups are shown by “,” which indicates ; (b) PLK mRNA expression after mir3686 transfected PANC1 cells. Cells transfected with mir3686 or scramble control for 48 h then the expression of PLK1 mRNA were detected by real-time PCR in different groups. Three repeating experiments had been conducted for each group. Significant differences between groups are shown by “.” (c) Expression of PLK1 protein in PANC1 transfected with mir3686 and scramble. The Western blotting was done with anti-PLK-1 antibody.

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