Regulation of Angiogenic Functions by Angiopoietins through Calcium-Dependent Signaling Pathways

Figure 2

Ang-1 and Ang-2 mobilize from ER but not from acidic stores. Live imaging in Fura-2-AM-loaded single cells. Identification of Ang-1- and Ang-2-activated stores. Histograms represent Ca²⁺ release in cells stimulated with 100 ng/mL Ang-1 or 200 ng/mL Ang-2 after pretreatment with vehicle alone, or with 1 μM thapsigargin for 15 min (a, c), or with 500 nM bafilomycin A1 for 1 h in Ca²⁺-free medium KHH (b, d). Δ on the -axes = nM. Data in bar charts represent mean ± s.e.m. from three independent experiments. Cell number = 68–187. by Student’s -test.

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