Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design

Figure 7

Histopathologic, ultrastructural (EM), and immunohistochemical findings in a prairie dog that succumbed to Congo Basin MPXV (PD32). (a) Liver showing prominent inclusions (arrows) as seen by IHC staining of Orthopoxvirus antigens. (b) Thin section EM of liver showing oval and brick shaped monkeypox virions, with dense dumb-bell shaped cores within hepatocytes. (c) Urinary bladder showing desquamated epithelial lining cells (arrows) containing poxvirus antigens as seen by IHC. (d) Submandibular lymph node showing abundant macrophages and vascular (arrows) poxvirus antigens seen by IHC. (e-f) Orthopoxvirus IHC showing typical intracytoplasmic inclusions (arrows) by H&E (e) and extensive staining of orthopoxvirus antigens as seen by IHC (f) within the oropharynx.

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