Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design

Figure 8

Apoptosis within MPXV infected prairie dog liver and spleen. Prairie dogs intranasally challenged (8 × 10³ p.f.u.) with West African WA MPXV were serially sacrificed (days 2, 4, 6, 9, 12, 17, and 24) following challenge. Representative images of immunohistochemical staining of paraffin-embedded prairie dog liver (a, b, c) and spleen (d, e, f) sections stained with Millipore Apoptosis kit. Apoptotic cells are identified by red-brown staining nucleus. (400x original magnification, scale bar = 20 μm, immunoperoxidase method, Vector NovaRed chromogen, hematoxylin counterstain).