Competing Role of Bioactive Constituents in Moringa oleifera Extract and Conventional Nutrition Feed on the Performance of Cobb 500 Broilers

Table 3

LC-MS/MS-compound identification.

(a) Compounds identified from the chromatogram of 0.5% w/w Moringa oleifera leaves extracts

Peak	Retention time (RT)	Molecular ion peak (M–H)^−	MS^2 fragment ions intensity	Tentative compounds identified
1	0.52	117	73 (100), 99, 72	Succinic acid conjugate
2	0.65	164	147 (100), 119, 103, 72	Cinnamic acid conjugate
3	0.92	203	116 (100), 142, 159	Tryptophan
4	1.19	337	191, 163, 119 (100)	4-p-Coumaroylquinic acid
5	1.85	593	383, 353 (100), 325, 297	Apigenin-6,8-di-C-β-D-glucopyranoside
6	2.24	432	284 (100), 283, 312, 269	Apigenin 6 C glucoside (isovitexin)
7	2.36	252	208, 151, 164, 107 (100)	Zeaxanthin derivative
8	2.78	266	222, 178, 170 (100), 151	Unknown
9	3.04	301	257, 108 (100), 109, 65	Quercetin
10	3.73	330	294, 230, 224, 212 (100)	3,30-di-O-Methyl ellagic acid conjugate
11	4.31	309	291, 263, 251, 247, 211, 197 (100), 171	Unknown
12	5.03	481	283, 255 (100), 224, 168, 153	Kaempferol derivative
13	5.42	480	285, 255 (100), 242, 224, 168	Kaempferol derivative
14	8.07	856	596, 575, 431, 297, 279 (100), 241, 153	Inositol derivatives
15	8.72	739	279 (100), 241, 153
16	10.04	858	279 (100), 241, 153
17	10.7	832	277, 255 (100), 241, 153

(b) Compounds identified from the chromatogram of 0.5% w/w Moringa oleifera leaves extracts with nutritional feed

Peak	Retention time (RT)	Molecular ion peak (M–H)^−	MS^2 fragment ions intensity	Tentative compounds identified
1	1.18	253	223, 180, 81 (100), 80	Diazin
2	1.85	253	223 (100), 208, 180, 132, 81	Daidzein
3	2.37	269	159, 133, 65 (100), 63	Genistein
4	2.65	253	223 (100), 208, 180, 167, 133	Formononetin
5	3.00	269	159, 134, 65 (100), 63	Genistein
6	3.83	376	296, 278, 192, 133, 80 (100)	Unknown
7	4.22	393	329 (100), 301, 224, 209, 183	Ellagic acid conjugate
8	4.75	313	295, 283, 201, 183 (100)	Dimethoxyflavone conjugate
9	5.57	295	277 (100), 233, 183, 195, 171	Unknown
10	6.08	572	315, 255 (100), 241, 153	Lysophosphatidylinositols
11	6.38	299	297, 282 (100), 255	Methyl stearate
12	7.14	280	279 (100), 261, 244	Linoleic acid
13	7.68	281	256 (100), 255	Oleic acid
14	8.65	283	281 (100), 253	Stearic acid
15	9.5	382	311 (100), 255, 171, 125	Methyl ester fatty acids
16	10.5	364	301, 255, 171 (100)	Quercetin
17	10.7	384	311 (100), 255, 169, 125	Methyl ester fatty acids