Inhibition of PI3K Signalling Selectively Affects Medulloblastoma Cancer Stem Cells

Figure 3

PI3K/AKT inhibition induces strong apoptosis in MDB primary cells. (a) Bar graph reporting data from Annexin-V/PI analyses performed on primary MDB cells (HuTuP33, HuTuP49, and FI25) after LY294002 (LY) treatment (15 μM). (b) Western blot analysis performed on FI25 primary MDB cells showing a dramatic induction of the apoptotic process after LY294002 treatment. β-actin levels have been used as loading control. (c, d): TUNEL (green) cell death analysis performed on MDB primary cells (FI25 and HuTuP33), showing a significant increase of cell death after 72 h of LY294002 treatment (c) and relative quantification (d). Original magnification 20x, bar: 50 μm. For all graphs, values are represented as mean of at least 3 independent experiments ± S.E.M. and .

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