Inhibition of PI3K Signalling Selectively Affects Medulloblastoma Cancer Stem Cells

Figure 4

PI3K/AKT inhibition promotes neuronal differentiation of MDB primary cells. (a, b): representative images (FI25) (a) and relative quantification (FI25 and HuTuP33) (b) of percentages of cells positive for Nestin (green) and/or βIII-tubulin (red) at different time points from LY294002 (LY) treatment. Original magnification 20x, bar: 20 μm. (c) Western blot analysis reporting HIF-1α levels after PI3K/AKT inhibition mediated by LY294002. (d) Bar graph showing CD133⁺ cells decrease after LY294002 treatment (HuTuP33, HuTuP49, and FI25). For all graphs, mean of 3 independent experiments ± S.E.M. .

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