Inhibition of PI3K Signalling Selectively Affects Medulloblastoma Cancer Stem Cells

Figure 5

PI3K/AKT inhibition induces significant cell death selectively in CD133⁺ MDB primary cells. (a) Bar graph comparing LY294002- (LY-) induced cell death between CD133⁺ MDB primary cells (sorted) and the total cell population by Annexin-V staining. CD133⁺ cells demonstrate a significant () increase of apoptosis levels. (b–d) Cytofluorimetric analysis of MDB primary cells showing CD133⁺ cell subpopulation (orange label) is more sensitive to LY294002 treatment than CD133⁻ cells (black label) as shown by Annexin-V staining in (d). Representative dot plots are reported for cytofluorimetric analysis of HuTuP33 MDB cells. For all graphs, mean of 3 independent experiments ± S.E.M. in the comparison with control cells.

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