Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight

Figure 2

Biological processes enriched amongst genes associated with flight-specific fitness defects at different time points in the homozygous deletion series. Each node represents a significantly enriched gene ontology (GO) biological process (hypergeometric test ). A circle node indicates enrichment at 14 generations compared to 7 generations (the first time point), a square node indicates enrichment at 21 generations compared to 7 generations, and a diamond node indicates enrichment at both 14 generations and 21 generations (see Methods). Node size is proportional to the significance of enrichment [−log₁₀()]. Node color indicates processes that share genes (see Methods) and summary labels are shown for nodes of the same color. Edges indicate ≥ 50% gene overlap between connected processes; width is proportional to the degree of overlap. Each bar plot provides fitness defect (FD) scores for genes that contribute to the enrichment of processes with the same node color as the plot border. Specifically, the length of a bar is proportional to the log₂(abundance_7G/abundance_14G/21G), where abundance_yG represents the abundance of the corresponding gene deletion strain at generations (see Methods). An “” on the bar indicates that the abundance of the strain lowers to background level at later time point.