Active Microbial Communities Inhabit Sulphate-Methane Interphase in Deep Bedrock Fracture Fluids in Olkiluoto, Finland

Table 4

The number of sequence reads, the observed and estimated number of OTUs, diversity coverage, and diversity index () obtained by the HTP sequencing of bacterial and archaeal 16S rRNA and dsrB and mcrA transcripts. The diversity and OTU richness estimates were calculated based on equal number of sequence reads.

		296 m	328 m	347 m	415 m	559 m	572 m	798 m
Bacteria 16S	Number of reads	1220	893	9209	18425	17158	5377	996
	Observed OTUs	45	46	35	63	83	169	49
	Estimated richness
	Chao	161	294	73	126	161	367	104
	Ace	412	355	111	219	355	587	139
	Coverage % chao	28	16	48	50	56	46	47
		1.67	1.25	0.15	0.44	1.79	0.17	1.43
Archaea 16S	Number of reads	6322	12785	1377	2122	1223	1655	3277
	Observed OTUs	139	67	32	45	26	26	6
	Estimated richness
	Chao	249	137	47	46	46	28	7
	Ace	382	210	81	60	75	30	16
	Coverage % chao	56	49	68	98	57	93	86
	^*	1.06	0.81	1.91	1.23	0.80	1.04	0.83
dsrB	Number of reads	8131	4144	12649	8628	2360	2249	—
	Observed OTUs	33	41	47	50	13	26
	Estimated richness
	Chao	38	42	51	60	16	31
	Ace	39	45	52	63	38	49
	Coverage % chao	86.8	97.6	92.2	83.3	81.3	83.9
	^*	2.29	2.65	0.69	1.03	1.93	1.81
mcrA	Number of reads	—	4188	4184	—	—	6676	2324
	Observed OTUs		4	2			2	1
	Estimated richness
	Chao		4	2			2	1
	Ace		5	0			0	0
	Coverage % chao		100	100			100	100
	^*		0.45	0.42			0.76	0.43

Normalized according to sample with the lowest number of reads.