Pathogenic Potential of Two Sibling Species, Anisakis simplex (s.s.) and Anisakis pegreffii (Nematoda: Anisakidae): In Vitro and In Vivo Studies

Table 3

Survival rate (%) of Anisakis  simplex (s.s.) and Anisakis  pegreffii immersed in 2 different solutions. Data are expressed as the mean survival rate of triplicates ± standard errors.

Species	Days after immersion	PBS^a	AGJ^b
Anisakis  simplex (s.s.)	1	100.0 ± 0.0	83.3 ± 12.0
	2	100.0 ± 0.0	83.3 ± 12.0
	3	83.3 ± 12.0	66.6 ± 6.7
	4	83.3 ± 12.0	56.7 ± 6.7
	5	80.0 ± 15.3	50.0 ± 5.8
	6	80.0 ± 15.3	36.7 ± 3.3
	7	80.0 ± 15.3	3.33 ± 3.3
Anisakis  pegreffii	1	100.0 ± 0.0	100.0 ± 0.0
	2	100.0 ± 0.0	66.7 ± 3.3
	3	100.0 ± 0.0	33.3 ± 3.3
	4	100.0 ± 0.0	20.0 ± 5.8
	5	100.0 ± 0.0	6.7 ± 6.7
	6	96.7 ± 3.3	3.3 ± 3.3
	7	96.7 ± 3.3	0.0 ± 0.0

^aPBS: phosphate buffered saline, pH 7.2.
^bAGJ: artificial gastric juice, pH 1.8.