Pathogenic Potential of Two Sibling Species, Anisakis simplex (s.s.) and Anisakis pegreffii (Nematoda: Anisakidae): In Vitro and In Vivo Studies

Table 4

The locations of Anisakis  simplex (s.s.) and Anisakis  pegreffii in rats after necropsy. Means in each column with the same superscript lower-case letters (a–c for A.  simplex (s.s.) and d–f for A.  pegreffii) are not significantly different (). Means in each row with the same subscript upper-case letters (A–C for A.  simplex (s.s.) and D–F for A.  pegreffii) are not significantly different ().

Species	Time after challenge	Number of rats	Number of administered larvae	% of recovered larvae/administered larvae						Number of recovered larvae/administered larvae (%)
				Esophagus	Stomach wall (inner)	Stomach	Stomach wall (outer)	Intestines	Abdominal cavity
Anisakis  simplex (s.s.)	3 h	3	30	0.0 ± 0.0	6.7 ± 6.7	60.0 ± 11.6	0.0 ± 0.0	10.0 ± 5.8	10.0 ± 10.0	86.7 ± 13.3
	6 h	3	30	3.3 ± 3.3	3.3 ± 3.3	40.0 ± 15.3	0.0 ± 0.0	23.3 ± 14.5	6.7 ± 3.3	76.7 ± 6.7
	12 h	3	30	0.0 ± 0.0	3.3 ± 3.3	20.0 ± 10.0	0.0 ± 0.0	16.7 ± 8.8	0.0 ± 0.0	40.0 ± 20.8
	24 h	3	30	0.0 ± 0.0	10.0 ± 5.8	0.0 ± 0.0	0.0 ± 0.0	6.7 ± 3.3	3.3 ± 3.3	20.0 ± 5.8
Anisakis  pegreffii	3 h	3	30	0.0 ± 0.0	3.3 ± 3.3	50.0 ± 15.3	13.3 ± 8.8	3.3 ± 3.3	3.3 ± 3.3	73.3 ± 8.8
	6 h	3	30	0.0 ± 0.0	6.7 ± 3.3	53.3 ± 14.5	3.3 ± 3.3	10.0 ± 5.8	0.0 ± 0.0	73.3 ± 17.6
	12 h	3	30	0.0 ± 0.0	20.0 ± 10.0	30.0 ± 11.6	0.0 ± 0.0	6.7 ± 3.3	10.0 ± 5.8	66.7 ± 3.3
	24 h	3	30	0.0 ± 0.0	6.7 ± 3.3	3.3 ± 3.3	6.7 ± 3.3	0.0 ± 0.0	36.7 ± 23.3	53.3 ± 23.3