Dietary Flavonoid Hyperoside Induces Apoptosis of Activated Human LX-2 Hepatic Stellate Cell by Suppressing Canonical NF-κB Signaling

Figure 5

Hyperoside induces HSC apoptosis by blocking NF-κB activation and mediating NF-κB-dependent genes. (a) Hyperoside blocks NF-κB activation in Lx-2 cells. The effect of hyperoside on NF-κB DNA-binding activity in LX-2 cells was evaluated by ELISA. LX-2 cells were incubated for 48 hours in the absence or presence of TNF-α (20 ng/mL) and hyperoside (1 mM/L). Nuclear protein was extracted and subjected to ELISA for measurement of NF-κB DNA-binding activity. (b) Relative expression of NF-κB mediating proapoptotic target genes. (c) Relative expression of NF-κB mediating antiapoptotic target genes ( compared with the control group, ; compared with the control group, ,   ).

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