Methylation Status of Alu and LINE-1 Interspersed Repetitive Sequences in Behcet’s Disease Patients

Figure 1

LINE-1 and Alu methylation analysis using COBRA. Restriction enzyme TaqI cuts after methylated cytosine residues (represented by closed circles) and enzyme TasI cuts after unmethylated cytosine residues (represented by open circles). (a) Bisulfite treated DNA was amplified to generate LINE-1 amplicons and then digested with TaqI and TasI enzymes to obtain fragments representing the four possible methylation patterns (, , , and ), producing bands at 92, 60, 50, and 18 bp, respectively. (b) Bisulfite treated DNA was amplified to generate Alu amplicons and then digested with TaqI to obtain fragments representing the four possible methylation patterns (, , , and ), producing bands at 133, 90, 75, and 32 bp, respectively.

(a)

(b)