TRIM25 Identification in the Chinese Goose: Gene Structure, Tissue Expression Profiles, and Antiviral Immune Responses In Vivo and In Vitro

Figure 6

Effects of H9N2, GPV, poly(I:C), R848, ODN2006, and goose IFNα on goose TRIMM25 transcript levels in PBMCs. β-Actin was used as the internal control gene to detect the transcription levels of goose TRIM25. PBMCs were infected with either H9N2 or GPV for 24 hours. PBMCs were treated with poly(I:C), ODN2006, R848 for 6 hours, and IFNα for 24 hours. The expression data represent the mean ± SEM (). The differences in mRNA cytokine production in virus-challenged goslings and cells were analyzed using the unpaired, two-tailed -test ().