Selection of Lipases for the Synthesis of Biodiesel from Jatropha Oil and the Potential of Microwave Irradiation to Enhance the Reaction Rate
Table 2
Catalytic properties of the lipase preparations used in this study (crude extracts and immobilized on epoxy-SiO2-PVA).
Lipase source
Designation
Crude lipases
Immobilized lipases
Protein (mg/g)
Hydrolytic activity (IU/g)
Specific activity (IU/mg of protein)
Hydrolytic activity (IU/g of support)
Specific activity (IU/)
Aspergillus niger
Lipase A
96.3 ± 1.3
23750.9 ± 370.2
246.6 ± 15.4
756.8 ± 21.4
31.4 ± 0.9
Mucor javanicus
Lipase M
121.4 ± 3.7
40875.4 ± 1220.8
337.8 ± 18.3
1286.3 ± 77.3
42.5 ± 2.5
Rhizopus oryzae
Lipase L036P
93.5 ± 1.9
45112.4 ± 907.7
484.1 ± 21.9
2925.4 ± 100.8
125.0 ± 4.3
Rhizopus oryzae
Piccantase R8000
133.9 ± 2.4
13848.1 ± 268.7
104.2 ± 7.3
749.1 ± 51.7
22.4 ± 1.5
Burkholderia cepacia
Lipase PS
13.0 ± 0.3
30422.8 ± 1044.5
5416.7 ± 102.6
1980.6 ± 88.4
609.4 ± 27.2
Pseudomonas fluorescens
Lipase AK
14.2 ± 0.4
29878.5 ± 890.6
2133.9 ± 87.5
1860.3 ± 94.5
523.9 ± 26.7
Porcine pancreatic
Pancreatic
121.8 ± 2.1
19802.7 ± 371.4
162.3 ± 12.4
1059.8 ± 42.9
34.8 ± 1.4
Protein concentration determined by the Bradford method. Hydrolytic activity measured on the olive oil emulsion hydrolysis (pH 7.0, 37°C). Calculated as the hydrolytic activity of the enzyme per milligram of protein. Calculated as the hydrolytic activity of the enzyme per milligram of immobilized protein (IP).
