The Effect of Adipose-Derived Stem Cells on Full-Thickness Skin Grafts

Figure 3

Anti-inflammatory effect of ASCs by converting macrophage to M2 polarization. (a) Proinflammatory cytokines (IL-1, IL-6, and TNF-α), anti-inflammatory cytokines (IL-10), and M1, M2 macrophages surface marker iNOS and Arg-1, CD206, and CD163 expression in skin grafts for various times. mRNA expression of interested cytokines is normalized to β-actin and given as relative change. Data are presented as the mean ± SD of three separate experiments. with PBS, . (b) Immunofluorescence analysis of M2 phenotype on days 1 and 2. The densities and distributions of M2 macrophages were significantly increased in the ASC group especially on day 2. Scale bar 100 μm. (c) The protein level of Arg-1 at days 1 and 2 by western blotting.

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