A Novel Murine Model of Parvovirus Associated Dilated Cardiomyopathy Induced by Immunization with VP1-Unique Region of Parvovirus B19

Table 1

Echocardiographic parameters of immunized animals.

Antigen	Days after immunization	LV end-diastolic diameter (mm)	LV end-systolic diameter (mm)	Fractional shortening (%)	LV ejection fraction (%)
		Mean ± SE	Mean ± SE	Mean ± SE	Mean ± SE
PBS	0	3.93 ± 0.10	2.64 ± 0.10	32.9 ± 1.3	61.9 ± 1.8
	20	4.12 ± 0.14	2.70 ± 0.14	34.7 ± 1.5	64.2 ± 2.1
	47	4.05 ± 0.10	2.66 ± 0.07	34.4 ± 1.3	64.0 ± 1.8
	60–69	4.25 ± 0.10	2.83 ± 0.19	33.6 ± 3.2	62.3 ± 4.2
Simple main effect for measurement time		(3, 9) = 1.025, = 0.426, partial = 0.255	(3, 9) = 0.574, = 0.646, partial = 0.161	(3, 9) = 1.010, = 0.432, partial = 0.252	(3, 9) = 1.060, = 0.413 ,; partial = 0.261
Cardiac myosin	0	3.83 ± 0.05	2.39 ± 0.09	37.5 ± 1.6	68.1 ± 2.1
	20	4.12 ± 0.10	2.98 ± 0.13	27.7 ± 1.8	54.0 ± 2.7
	47	4.46 ± 0.12	3.46 ± 0.11	22.6 ± 0.5	45.7 ± 0.9
	60–69	4.48 ± 0.04	3.57 ± 0.06	20.4 ± 0.9	41.7 ± 1.5
Simple main effect for measurement time		(3, 12) = 25.761, < 0.0005, partial = 0.866	(3, 12) = 37.638, < 0.0005, partial = 0.904	(3, 12) = 35.975, < 0.0005, partial = 0.900	(3, 12) = 36.057, < 0.0005, partial = 0.900
VP1u	0	3.71 ± 0.07	2.43 ± 0.07	34.5 ± 1.7	64.1 ± 2.4
	20	4.00 ± 0.06	2.77 ± 0.11	30.8 ± 20	58.7 ± 3.0
	47	4.33 ± 0.09	3.26 ± 0.05	24.7 ± 1.1	49.1 ± 1.8
	60–69	4.39 ± 0.14	3.21 ± 0.15	26.9 ± 1.5	52.6 ± 2.4
Simple main effect for measurement time		(3, 15) = 9.760, < 0.005, partial = 0.661	(3, 15) = 18.526, < 0.0005, partial = 0.787	(3, 15) = 13.968, < 0.0005, partial = 0.736	(3, 15) = 13.707, < 0.0005, partial = 0.733
Two-way interaction between immunization and the time on echocardiographic parameter		(6, 36) = 3.755, < 0.005, partial = 0.385	(6, 36) = 8.669, < 0.0005, partial = 0.591	(6, 36) = 9.225, < 0.0005, partial = 0.606	(6, 36) = 9.460, < 0.0005, partial = 0.612