Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells

Table 3

Primers for qRT-PCR validation.

Gene short name	Forward primer sequence 5′-3′	Reverse primer sequence 5′-3′
KRT19	GGTCATGGCCGAGCAGAA	TTCAGTCCGGCTGGTGAAC
DKK1	AGAAAAGGCTCTCATGGACTAGAAAT	CCGGCAAGACAGACCTTCTC
APOC3	TGGCTGCCTGAGACCTCAAT	AGGAGCTCGCAGGATGGATA
FGG	TGGTTGGTGGATGAACAAGTGT	TGCCACCTTGGTAATAAACTCCAT
PIFB2	CTGGATGTGGTAGTGAACTTGAGACT	ACGTGGTCCCCTGAAGCTT
SLC2A3	GGCACACGGTGCAGATAGATC	GCAGGCTCGATGCTGTTCAT
FGA	CAGATGAGGCCGGAAGTGA	GATTTAGCATGGCCTCTCTTGGT
FGB	CAGCCGGTAATGCCCTCAT	TGTGAATGGTCATGGTCCTGTT
CLDN6	CTTGGATGATGGAGCCAAAGA	TGGCTTCTAAGATGGGCATGT
SFN	GCAGGCCGAACGCTATGA	TCCACGGCGCCTTTCA
FREM1	ACCTGGGCAACCTTGTAACTGTA	TGGTCGTTCAAACCTATCCAAA
ALCAM	CAATGCCCCAAACTTTCTCATAA	TGTCCCCAATCTTCACAAGCT
YLPM1	GGAAACTGCACCTCGTCACA	GCAGCATCTTGCAGCAAAGA
SCARA3	CCCTGAGAAGTTCAACATTTATTTCTT	GGGCAGAGGCAAGGATGAAT
NLRP1	CCCTCTATCGGCGTCTATCTGT	GCTCTTACCGTCTCTTATTCAGCAT
S100A4	CGCCAGTGGGCACTTTTTT	CAGCATCAAGCACGTGTCTGA
PTGR1	AAGAAATTTGGAAGGATTGCCATA	GAAGTGGGCCGGTTCTGTTA
NUPR1	GGGTGGCAGCAACAATAAATAGA	GGATGAACACACACCCAAGCT