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BioMed Research International
Volume 2016, Article ID 1801646, 8 pages
Research Article

Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley

1Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
2Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China

Received 21 January 2016; Revised 30 May 2016; Accepted 8 June 2016

Academic Editor: Atanas Atanassov

Copyright © 2016 Ruiju Lu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would significantly decrease the callus induction. And the further optimization experiments revealed that the increasing of organic nitrogen sources was much important in callus induction while it seemed different in plant regeneration. Based on the great effects of organic nitrogen on callus induction, the medium of N6-ANO1/4-2000 might be the best choice for the microspore culture system. In addition, the phylogenetic analysis indicated that there were clear differences of genetic backgrounds among these barley genotypes, and it also suggested that this medium for microspore culture had widespread utilization in different barley genotypes.