Research Article

Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley

Table 1

Culture media within different nitrogen sources.

Induction mediaNotesAmmonia nitrogen
(NH4)2SO4 (mg/L)
Nitrate nitrogen
KNO3 (mg/L)
Organic nitrogen
Glutamine (mg/L)Casein hydrolysate (mg/L)

N6-ANON6 medium supplemented with ammonia, nitrate, and organic nitrogen sources463.02830.0800800

N6-AN6 medium supplemented only with ammonia nitrogen463.0000

N6-NN6 medium supplemented only with nitrate nitrogen02830.000

N6-ON6 medium supplemented with two organic nitrogen sources00800800

N6-ANN6 medium supplemented with both ammonia and nitrate463.02830.000

N6-AON6 medium supplemented with ammonia and two organic nitrogen sources463.00800800

N6-NON6 medium supplemented with nitrate and two organic nitrogen sources02830.0800800

N6-ANO3/4N6 medium supplemented with 3/4 of nitrogen sources of N6-ANO347.32122.5600600

N6-ANO1/2N6 medium supplemented with 1/2 of nitrogen sources of N6-ANO231.51415.0400400

N6-ANO1/4N6 medium supplemented with 1/4 of nitrogen sources of N6-ANO115.8707.5200200

N6N6 medium supplemented without any nitrogen0000

N6-ANO1/4-2000N6 medium supplemented with 1/4 levels of inorganic nitrogen sources of N6-ANO, plus 2000 mg of each organic nitrogen115.8707.520002000

N6-ANO1/4-1600N6 medium supplemented with 1/4 levels of inorganic nitrogen sources of N6-ANO, plus 1600 mg of each organic nitrogen115.8707.516001600

N6-ANO1/4-1200N6 medium supplemented with 1/4 levels of inorganic nitrogen sources of N6-ANO, plus 1200 mg of each organic nitrogen115.8707.512001200

N6-ANO1/4-800N6 medium supplemented with 1/4 levels of inorganic nitrogen sources of N6-ANO, plus 800 mg of each organic nitrogen115.8707.5800800