BioMed Research International / 2016 / Article / Fig 2

Review Article

Plant Responses to High Frequency Electromagnetic Fields

Figure 2

Effect of calcium concentration in culture medium on calm-n6 (calmodulin), pin2 (proteinase inhibitor), and lecdpk1 (calcium-dependent protein kinase) transcript accumulation in response to the HF-EMF exposure. (a) Standard medium (0.73 mM of calcium). (b) Tenfold extra calcium (7.3 mM). (c) No calcium (0 mM). (d) No calcium (0 mM) with 0.5 mM of EGTA. (e) No calcium (0 mM) with 0.4 mM of BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid), a specific Ca2+ chelator. (f) No calcium (0 mM) with 0.1 mM LaCl3. Bars represent mean values ± SE from at least three independent experiments. An asterisk over the bars states the significant differences according to the one-sided Mann-Whitney test. Reproduced from [10], with permission.
(a) Standard (0.73 mM calcium)
(b) Standard + calcium (7.3 mM)
(c) Standard − calcium (0 M)
(d) EGTA (0.5 mM)
(e) BAPTA (0.4 mM)
(f) LaCl3 (0.1 mM)

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