A Tobacco-Derived Thymosin β4 Concatemer Promotes Cell Proliferation and Wound Healing in Mice

Figure 5

Tobacco-derived 4×Tβ4 promotes keratinocyte migration (a) and formation of blood vessels in the wound beds of mice (b). (A) Tobacco-derived 4×Tβ4 protein (5 μg), (B) tobacco-derived 4×Tβ4 protein (2.5 μg), (C) TSP (total soluble protein) (50 μL) extracted from untransformed tobacco leaves, (D) 50 μL PBS extraction buffer, (E) E. coli-derived 4×Tβ4 protein (5 μg), and (F) commercially purchased standard Tβ4 (5 μg). Black arrows indicate newly formed blood vessels and reepithelialization of the wound epidermis following topical treatments. Scale bars = 50 μm.

(a)

(b)