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BioMed Research International
Volume 2016 (2016), Article ID 2043787, 10 pages
http://dx.doi.org/10.1155/2016/2043787
Research Article

Optimization of Cultural Conditions for Antioxidant Exopolysaccharides from Xerocomus badius Grown in Shrimp Byproduct

1School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China
2Department of Biological & Agricultural Engineering, North Carolina State University, Raleigh, NC 27695, USA

Received 15 November 2015; Revised 15 January 2016; Accepted 18 January 2016

Academic Editor: Gjumrakch Aliev

Copyright © 2016 Xiujun Gao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To optimize the production conditions for exopolysaccharides with higher antioxidant activities from Xerocomus badius cultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of  g/L and a total antioxidant activity of  U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).