Research Article

Optimization of Cultural Conditions for Antioxidant Exopolysaccharides from Xerocomus badius Grown in Shrimp Byproduct

Table 1

Plackett-Burman experimental design matrix for screening the cultural medium and conditions with mycelium biomass and yield of crude EPS as response.

RunsExperimental factorsYields of EPS (g/L)T-AO activity (U/mg)

11 (10.0)−1 (5.0)1−1 (100)1 (10.0)1−1 (120)−1 (1.30)−11 (30)1 (96)3.634 ± 0.1242.146 ± 0.130
21 (10.0)−1 (5.0)1−1 (100)−1 (8.0)−11 (150)1 (1.63)11 (30)−1 (72)1.956 ± 0.1711.371 ± 0.098
3−1 (8.0)1 (6.5)−1−1 (100)−1 (8.0)11 (150)−1 (1.30)11 (30)1 (96)1.724 ± 0.1630.864 ± 0.074
4−1 (8.0)−1 (5.0)11 (125)1 (10.0)−11 (150)−1 (1.30)1−1 (25)1 (96)2.135 ± 0.1741.776 ± 0.106
51 (10.0)−1 (5.0)−11 (125)−1 (8.0)1−1 (120)1 (1.63)1−1 (25)1 (96)1.481 ± 0.1331.542 ± 0.095
6−1 (8.0)−1 (5.0)−11 (125)1 (10.0)11 (150)1 (1.63)−11 (30)−1 (72)1.519 ± 0.1471.557 ± 0.107
71 (10.0)1 (6.5)−1−1 (100)1 (10.0)−11 (150)1 (1.63)−1−1 (25)1 (96)0.935 ± 0.1310.785 ± 0.034
81 (10.0)1 (6.5)−11 (125)1 (10.0)−1−1 (120)−1 (1.3)11 (30)−1 (72)2.493 ± 0.1952.217 ± 0.112
9−1 (8.0)−1 (5.0)−1−1 (100)−1 (8.0) −1−1 (120)−1 (1.30)−1−1 (25)−1 (72)2.061 ± 0.1441.563 ± 0.083
101 (10.0)1 (6.5)11 (125)−1 (8.0)11 (150)−1 (1.30)−1−1 (25)−1 (72)1.664 ± 0.2091.782 ± 0.091
11−1 (8.0)1 (6.5)1−1 (100)1 (10.0)1−1 (120)1 (1.63)1−1 (25)−1 (72)0.091 ± 0.0100.637 ± 0.047
12−1 (8.0)1 (6.5)11 (125)−1 (8.0)−1−1 (120)1 (1.63)−11 (30)1 (96)0.704 ± 0.0170.345 ± 0.022

Notes: , solid-to-liquid ratio (%, m/v); , bran powder content (%); , volume of liquid medium (mL); , inoculation amount of mycelium (%); , rotation rate (rpm); , concentration of glacial acetic acid (%); , cultural temperature (°C); , cultivation time (h); , , and refer to dummy columns.