Optimization of Cultural Conditions for Antioxidant Exopolysaccharides from Xerocomus badius Grown in Shrimp Byproduct
Table 1
Plackett-Burman experimental design matrix for screening the cultural medium and conditions with mycelium biomass and yield of crude EPS as response.
Runs
Experimental factors
Yields of EPS (g/L)
T-AO activity (U/mg)
1
1 (10.0)
−1 (5.0)
1
−1 (100)
1 (10.0)
1
−1 (120)
−1 (1.30)
−1
1 (30)
1 (96)
3.634 ± 0.124
2.146 ± 0.130
2
1 (10.0)
−1 (5.0)
1
−1 (100)
−1 (8.0)
−1
1 (150)
1 (1.63)
1
1 (30)
−1 (72)
1.956 ± 0.171
1.371 ± 0.098
3
−1 (8.0)
1 (6.5)
−1
−1 (100)
−1 (8.0)
1
1 (150)
−1 (1.30)
1
1 (30)
1 (96)
1.724 ± 0.163
0.864 ± 0.074
4
−1 (8.0)
−1 (5.0)
1
1 (125)
1 (10.0)
−1
1 (150)
−1 (1.30)
1
−1 (25)
1 (96)
2.135 ± 0.174
1.776 ± 0.106
5
1 (10.0)
−1 (5.0)
−1
1 (125)
−1 (8.0)
1
−1 (120)
1 (1.63)
1
−1 (25)
1 (96)
1.481 ± 0.133
1.542 ± 0.095
6
−1 (8.0)
−1 (5.0)
−1
1 (125)
1 (10.0)
1
1 (150)
1 (1.63)
−1
1 (30)
−1 (72)
1.519 ± 0.147
1.557 ± 0.107
7
1 (10.0)
1 (6.5)
−1
−1 (100)
1 (10.0)
−1
1 (150)
1 (1.63)
−1
−1 (25)
1 (96)
0.935 ± 0.131
0.785 ± 0.034
8
1 (10.0)
1 (6.5)
−1
1 (125)
1 (10.0)
−1
−1 (120)
−1 (1.3)
1
1 (30)
−1 (72)
2.493 ± 0.195
2.217 ± 0.112
9
−1 (8.0)
−1 (5.0)
−1
−1 (100)
−1 (8.0)
−1
−1 (120)
−1 (1.30)
−1
−1 (25)
−1 (72)
2.061 ± 0.144
1.563 ± 0.083
10
1 (10.0)
1 (6.5)
1
1 (125)
−1 (8.0)
1
1 (150)
−1 (1.30)
−1
−1 (25)
−1 (72)
1.664 ± 0.209
1.782 ± 0.091
11
−1 (8.0)
1 (6.5)
1
−1 (100)
1 (10.0)
1
−1 (120)
1 (1.63)
1
−1 (25)
−1 (72)
0.091 ± 0.010
0.637 ± 0.047
12
−1 (8.0)
1 (6.5)
1
1 (125)
−1 (8.0)
−1
−1 (120)
1 (1.63)
−1
1 (30)
1 (96)
0.704 ± 0.017
0.345 ± 0.022
Notes: , solid-to-liquid ratio (%, m/v); , bran powder content (%); , volume of liquid medium (mL); , inoculation amount of mycelium (%); , rotation rate (rpm); , concentration of glacial acetic acid (%); , cultural temperature (°C); , cultivation time (h); , , and refer to dummy columns.