Improved Stability of a Model IgG3 by DoE-Based Evaluation of Buffer Formulations

Table 2

Full factorial DoE for dual buffer component (His/Arg) formulations. Detailed composition of each buffer tested in the 2⁴ full factorial DoE.

	Pattern	Arginine (mM)	Histidine (mM)	NaCl (mM)	pH
HR 1	++−+	200	50	50	6.5
HR 2	+−+−	200	25	100	6.0
HR 3	++−−	200	50	50	6.0
HR 4	++++	200	50	100	6.5
HR 5	−−−−	100	25	50	6.0
HR 6	−+++	100	50	100	6.5
HR 7	−+−+	100	50	50	6.5
HR 8	−++−	100	50	100	6.0
HR 9	−−+−	100	25	100	6.0
HR 10	−+−−	100	50	50	6.0
HR 11	−−−+	100	25	50	6.5
HR 12	+−++	200	25	100	6.5
HR 13	+−−+	200	25	50	6.5
HR 14	+++−	200	50	100	6.0
HR 15	−−++	100	25	100	6.5
HR 16	+−−−	200	25	50	6.0