Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture

Table 4

COL2a1 gene expression quantified by real-time PCR during organ culture.

Culture time point	Day 0	Day 3	Day 7	Day 14
Static load	1.000 0.038	1.195 0.040	0.016 0.002	0.005 0.001

The values represent the means ± SD. Significance of differences between culture time point and fresh tissue (day 0); significance of differences between culture time point and day 3. COL2a1: collagen type II; PCR: polymerase chain reaction; SD: standard deviation.