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BioMed Research International
Volume 2016, Article ID 2483258, 11 pages
http://dx.doi.org/10.1155/2016/2483258
Research Article

Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana

1Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
2Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
3Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
4Guangxi Academy of Agricultural Sciences, Nanning 530007, China
5Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia
6State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000, China

Received 5 February 2016; Accepted 20 April 2016

Academic Editor: Byoung R. Jeong

Copyright © 2016 Ming Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Water channel proteins, also called aquaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch. & C.A. Mey (C. bungeana) are still unknown. Results. In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C. bungeana. Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus. Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C. bungeana. Conclusion. This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C. bungeana. Our findings contribute to understanding the water transports in plants under low temperatures.