TY - JOUR
A2 - Umar, Sadiq
AU - Liu, Ya-Fei
AU - Wen, Cai-Yu-Zhu
AU - Chen, Zhe
AU - Wang, Yu
AU - Huang, Ying
AU - Tu, Sheng-Hao
PY - 2016
DA - 2016/09/05
TI - Effects of Berberine on NLRP3 and IL-1β Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation
SP - 2503703
VL - 2016
AB - Background. Urate crystals-induced inflammation is a critical factor during the initiation of gouty arthritis. Berberine is well known for its anti-inflammatory activity. However, the underlying effects of berberine on monosodium urate crystals-induced inflammation remain obscure. Objectives. This study is set to explore the protective effect and mechanism of berberine on monosodium urate crystals-induced inflammation in human monocytic THP-1 cells. Methods. The mRNA levels of NLRP3 and IL-1β were measured by Real-Time PCR, and the protein levels of NLRP3 and IL-1β were determined by ELISA, Western blot, and immunofluorescence. Results. The NLRP3 and IL-1β expressions were significantly increased in model group compared to that in normal group (P<0.05). Meanwhile, there was significant reduction in the expressions of NLRP3 and IL-1β mRNA in groups 6.25 μM berberine and 25 μM berberine when compared with model group (P<0.05). Conclusions. Therefore, berberine alleviates monosodium urate crystals-induced inflammation by downregulating NLRP3 and IL-1β expressions. The regulatory effects of berberine may be related to the inactivation of NLRP3 inflammasome.
SN - 2314-6133
UR - https://doi.org/10.1155/2016/2503703
DO - 10.1155/2016/2503703
JF - BioMed Research International
PB - Hindawi Publishing Corporation
KW -
ER -