Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing Bacterium Bacillus megaterium SP1 and Its Application in Aquaculture Wastewater Treatment
Table 3
The orthogonal experiment L16(45) of optimization of culture conditions.
A (g L−1)
B (g L−1)
C (°C)
D
E
Flocculating ratio
1
1 (10.0)
1 (0.2)
1 (20)
1 (7.0)
1
0.785
2
1
2 (0.5)
2 (25)
2 (6.5)
2
0.813
3
1
3 (0.8)
3 (30)
3 (6.0)
3
0.839
4
1
4 (1.0)
4 (35)
4 (5.5)
4
0.765
5
2 (15.0)
1
2
3
4
0.786
6
2
2
1
4
3
0.822
7
2
3
4
1
2
0.806
8
2
4
3
2
1
0.836
9
3 (20.0)
1
3
4
2
0.840
10
3
2
4
3
1
0.866
11
3
3
1
2
4
0.858
12
3
4
2
1
3
0.856
13
4 (25.0)
1
4
2
3
0.788
14
4
2
3
1
4
0.906
15
4
3
2
4
1
0.827
16
4
4
1
3
2
0.808
I
0.800
0.800
0.818
0.838
0.828
II
0.812
0.851
0.820
0.824
0.817
III
0.855
0.833
0.855
0.825
0.826
IV
0.832
0.816
0.806
0.813
0.829
R
0.055
0.052
0.049
0.025
0.002
Note. A: glucose; B: beef extract; C: culture temperature; D: medium initial pH; and E: blank control.
