The Subcellular Localization and Functional Analysis of Fibrillarin2, a Nucleolar Protein in Nicotiana benthamiana

Table 1

The sequences, homologous recombination, and restriction sites of PCR primers.

Primer and purpose	Sequence (5′→3′)	Modification
Construction for entry vector pDONR221
NbFib2-GF	ggggacaagtttgtacaaaaaagcaggcttcATGGTTGCACCAACTAGAGG	Homologous recombination
NbFib2-GR	ggggaccactttgtacaagaaagctgggtcGGCAGCAGCCTTCTGCTTCT	Homologous recombination
Construction for VIGS vector pYL156
NbFib2-VF	CGgaattcCGATGGTTGCACCAACTAGAGGTCGCG	EcoRI
NbFib2-VR	CGggatccCGTTAAATTTTCTAGGCAGCAGCCTTC	BamHI
Semiquantitative RT-PCR analysis of NbFib2 mRNA accumulation
NbFib2-F	ATGGTTGCACCAACTAGAGGTCGCG
NbFib2-R	GGCAGCAGCCTTCTGCTTCTTCCGGC
Actin-F	ACTGATGAAGATACTCACAGA
Actin-R	TGGAATTGTATGTGGTTTCAT

The lowercased letters indicate homologous recombination sequence or a restriction enzyme site.