Research Article
Effect of Phosphatase and Tensin Homologue on Chromosome 10 on Angiotensin II-Mediated Proliferation, Collagen Synthesis, and Akt/P27 Signaling in Neonatal Rat Cardiac Fibroblasts
Figure 6
Effect of PTEN on Ang II-induced (10−7 mM) Akt and P27 expression in CFs. After infection for 48 h, fibroblasts were treated with Ang II (10−7 mM) for 32 h. (a, b) P27 protein expression was assessed by western blotting. (c, d) Total Akt and phospho-Akt (pAkt-308) expression levels were assessed by western blotting. Each experiment was repeated three times with 3-4 replicates. , compared with the control Ad-GFP-infected and uninfected groups.