Phosphoprotein Phosphatase 1 Is Required for Extracellular Calcium-Induced Keratinocyte Differentiation

Figure 5

Calcium induces PP1 recruitment to E-cadherin in the plasma membrane. (a) Cultured human keratinocytes were treated with 1.2 mM calcium for 5 min. Cells were harvested and the protein levels of PP1α, PP1β, PP1γ, PIP5K1α, E-cadherin, integrin α2 (plasma membrane marker), BIP (endoplasmic reticulum marker), and GM130 (cis-Golgi marker) in total cell lysate and plasma membrane lysate were determined by western analysis. The results are from a representative experiment that was repeated three times. (b) The total cell lysates and the plasma cell lysates were isolated and analyzed by immunoprecipitation (IP) with antibody against E-cadherin followed by western analysis with antibodies against PP1α, PP1β, PP1γ, PIP5K1α, and E-cadherin.

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