Conditioning with Fludarabine-Busulfan versus Busulfan-Cyclophosphamide Is Associated with Lower aGVHD and Higher Survival but More Extensive and Long Standing Bone Marrow Damage

Figure 4

Expression of IFN-γ and IL-4 in serum on days +3, +5, +7, and +14 after allo-HSCT, proportion of CD3⁺ and CD19⁺ cells in bone marrow and CD4⁺ and CD8⁺ cells in spleen 14 days after transplantation. (a) INF-γ concentration in serum on days +3, +5, +7, and +14 after allo-HSCT. (b) IL-4 concentration in serum on days +3, +5, +7, and +14 after allo-HSCT (c) Proportion of CD4⁺ and CD8⁺ T-cells in spleen 14 days after allo-HSCT. (d, e) Proportion of CD3⁺ and donor-derived CD3⁺ T-cells and CD19⁺ and donor-derived CD19⁺ B-cells in bone marrow 14 days after allo-HSCT. Group A: normal BALB/c in the same condition. Group E: BALB/c conditioned with Bu (100 mg/kg)-Cy (200 mg/kg) and transplanted with bone marrow cells, spleen cells from C57BL/6. Group H: BALB/c conditioned with Flu (500 mg/kg)-Bu (100 mg/kg) and transplanted with bone marrow cells, spleen cells from C57BL/6. All data are presented as mean ± s.e. for 3–7 animals in each group per time point. ; ; NS, not significant, .

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