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BioMed Research International
Volume 2016, Article ID 3089584, 13 pages
Research Article

Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression in Dioscorea opposita

1College of Life Sciences, Henan Normal University, Xinxiang 453007, China
2Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, Xinxiang 453007, China
3Henan Province Engineering Laboratory of Green Medicinal Plant Biotechnology, Xinxiang 453007, China

Received 8 January 2016; Accepted 4 May 2016

Academic Editor: Young-Mi Lee

Copyright © 2016 Xiting Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most common methods for gene expression studies. Data normalization based on reference genes is essential for obtaining reliable results for qRT-PCR assays. This study evaluated potential reference genes of Chinese yam (Dioscorea opposita Thunb.), which is an important tuber crop and medicinal plant in East Asia. The expression of ten candidate reference genes across 20 samples from different organs and development stages was assessed. We identified the most stable genes for qRT-PCR studies using combined samples from different organs. Our results also suggest that different suitable reference genes or combinations of reference genes for normalization should be applied according to different organs and developmental stages. To validate the suitability of the reference genes, we evaluated the relative expression of PE2.1 and PE53, which are two genes that may be associated with microtuber formation. Our results provide the foundation for reference gene(s) selection in D. opposita and will contribute toward more accurate gene analysis studies of the genus Dioscorea.