Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples

Table 3

RPA assays were performed at the optimized target temperature of 39°C or at temperatures ±2°C of the target. Robust amplification was observed at all the temperatures tested.

Temperature (°C)	Onset time (min; )
37	6.3–7.3
38	5.7–6.7
39	5.7–6.3
40	5.3–6.0
41	5.3–5.7