Isolation and Physicochemical Characterization of Laccase from Ganoderma lucidum-CDBT1 Isolated from Its Native Habitat in Nepal

Figure 3

Optimization of experimental conditions for laccase production in Ganoderma lucidum-CDBT1. Modified Olga medium was used for G. lucidum-CDBT1 under various conditions shown in (a)–(d) as described in Materials and Methods. Experiments described in (a), (b), and (c) were performed at room temperature (~25°C). The modified Olga medium used to grow G. lucidum (experiments described in (a), (c), and (d)) contained 0.5 g/L CuSO₄ and 5 g/L lignin (experiments described in (b), (c), and (d)). (a) Effect of 5 g/L lignin on laccases secretion, (b) effect of CuSO₄ on laccase secretion, (c) effect of pH of the media on secretion of laccase, and (d) effect of incubation temperature on the secretion of laccases. Enzyme activity was determined as described in Materials and Methods. With the exception of the experiments involving the effect of media pH on secretion of laccase, all other experiments were performed at pH 5.0.

(a) Effect of lignin on laccase secretion

(b) Effect of CuSO₄ on laccase secretion

(c) Effect of pH on laccase secretion

(d) Effect of temperature on laccase secretion