Isolation and Physicochemical Characterization of Laccase from Ganoderma lucidum-CDBT1 Isolated from Its Native Habitat in Nepal

Table 1

Summary of purification of laccase from Ganoderma lucidum-CDBT1.

Enzyme	Total activity; (U)	Protein; (μg/mL)	Specific activity; (U/mg)	Fold-purification
Culture media-filtrate	97.22	105.7	0.92	1.0
Ammonium sulfate faction (after overnight dialysis)	3115.74	35.9	86.85	94.4
DEAE-anion exchange chromatography	2662.04	13.3	201.00	218.0

Preparation of the culture media-filtrate containing laccase and its ammonium sulfate fractionation and DEAE-anion exchange chromatography to isolate laccase were as described in Materials and Methods. Laccase enzyme was monitored using 1 mM ABTS as substrate at room temperature (~25°C) and protein concentrations in various factions were determined by Bradford assay described in Materials and Methods.