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BioMed Research International
Volume 2016, Article ID 3265895, 9 pages
http://dx.doi.org/10.1155/2016/3265895
Research Article

Improved Production of Aspergillus usamii endo-β-1,4-Xylanase in Pichia pastoris via Combined Strategies

Guangdong VTR Bio-Tech Co., Ltd., Zhuhai, Guangdong 519060, China

Received 11 November 2015; Accepted 24 January 2016

Academic Editor: Bidur P. Chaulagain

Copyright © 2016 Jianrong Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. Q. K. Beg, M. Kapoor, L. Mahajan, and G. S. Hoondal, “Microbial xylanases and their industrial applications: a review,” Applied Microbiology and Biotechnology, vol. 56, no. 3-4, pp. 326–338, 2001. View at Publisher · View at Google Scholar · View at Scopus
  2. M. L. T. M. Polizeli, A. C. S. Rizzatti, R. Monti, H. F. Terenzi, J. A. Jorge, and D. S. Amorim, “Xylanases from fungi: properties and industrial applications,” Applied Microbiology and Biotechnology, vol. 67, no. 5, pp. 577–591, 2005. View at Publisher · View at Google Scholar · View at Scopus
  3. A. C. E. Gregory, A. P. O'Connell, and G. P. Bolwell, “Xylans,” Biotechnology and Genetic Engineering Reviews, vol. 15, no. 1, pp. 439–456, 1998. View at Publisher · View at Google Scholar
  4. R. A. Prade, “Xylanases: from biology to biotechnology,” Biotechnology and Genetic Engineering Reviews, vol. 13, pp. 101–131, 1996. View at Google Scholar · View at Scopus
  5. S. Subramaniyan and P. Prema, “Biotechnology of microbial xylanases: enzymology, molecular biology, and application,” Critical Reviews in Biotechnology, vol. 22, no. 1, pp. 33–64, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. Y. Takahashi, H. Kawabata, and S. Murakami, “Analysis of functional xylanases in xylan degradation by Aspergillus niger E-1 and characterization of the GH family 10 xylanase XynVII,” SpringerPlus, vol. 2, article 447, 2013. View at Publisher · View at Google Scholar
  7. A. Hmida-Sayari, S. Taktek, F. Elgharbi, and S. Bejar, “Biochemical characterization, cloning and molecular modeling of a detergent and organic solvent-stable family 11 xylanase from the newly isolated Aspergillus niger US368 strain,” Process Biochemistry, vol. 47, no. 12, pp. 1839–1847, 2012. View at Publisher · View at Google Scholar · View at Scopus
  8. Y. Li, B. Zhang, X. Chen, Y. Chen, and Y. Cao, “Improvement of Aspergillus sulphureus endo-β-1,4-xylanase expression in Pichia pastoris by codon optimization and analysis of the enzymic characterization,” Applied Biochemistry and Biotechnology, vol. 160, no. 5, pp. 1321–1331, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. C. Zhou, J. Bai, S. Deng et al., “Cloning of a xylanase gene from Aspergillus usamii and its expression in Escherichia coli,” Bioresource Technology, vol. 99, no. 4, pp. 831–838, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. H.-M. Zhang, J.-Q. Wang, M.-C. Wu, S.-J. Gao, J.-F. Li, and Y.-J. Yang, “Optimized expression, purification and characterization of a family 11 xylanase (AuXyn11A) from Aspergillus usamii E001 in Pichia pastoris,” Journal of the Science of Food and Agriculture, vol. 94, no. 4, pp. 699–706, 2014. View at Publisher · View at Google Scholar · View at Scopus
  11. J. R. Wang, Y. Y. Li, S. D. Xu, P. Li, J. S. Liu, and D. N. Liu, “High-level expression of pro-form lipase from Rhizopus oryzae in Pichia pastoris and its purification and characterization,” International Journal of Molecular Sciences, vol. 15, no. 1, pp. 203–217, 2014. View at Publisher · View at Google Scholar
  12. D. Teng, Y. Fan, Y.-L. Yang, Z.-G. Tian, J. Luo, and J.-H. Wang, “Codon optimization of Bacillus licheniformisβ-1,3-1,4-glucanase gene and its expression in Pichia pastoris,” Applied Microbiology and Biotechnology, vol. 74, no. 5, pp. 1074–1083, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. R. Ramón, P. Ferrer, and F. Valero, “Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris,” Journal of Biotechnology, vol. 130, no. 1, pp. 39–46, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. X. Li, Z. Liu, G. Wang, D. Pan, L. Jiao, and Y. Yan, “Overexpression of Candida rugosa lipase Lip1 via combined strategies in Pichia pastoris,” Enzyme and Microbial Technology, vol. 82, pp. 115–124, 2016. View at Publisher · View at Google Scholar
  15. M. R. Yu, S. Wen, and T. W. Tan, “Enhancing production of Yarrowia lipolytica lipase Lip2 in Pichia pastoris,” Engineering in Life Sciences, vol. 10, no. 5, pp. 458–464, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. J. Wang, Y. Li, and D. Liu, “Gene cloning, high-level expression, and characterization of an alkaline and thermostable lipase from Trichosporon coremiiforme V3,” Journal of Microbiology and Biotechnology, vol. 25, no. 6, pp. 845–855, 2015. View at Publisher · View at Google Scholar
  17. J. R. Wang, Y. Y. Li, D. N. Liu et al., “Codon optimization significantly improves the expression level of α-amylase gene from Bacillus licheniformis in Pichia pastoris,” BioMed Research International, vol. 2015, Article ID 248680, 9 pages, 2015. View at Publisher · View at Google Scholar
  18. H. Y. Cai, P. J. Shi, Y. G. Bai et al., “A novel thermoacidophilic family 10 xylanase from Penicillium pinophilum C1,” Process Biochemistry, vol. 46, no. 12, pp. 2341–2346, 2011. View at Publisher · View at Google Scholar · View at Scopus
  19. X. Wang, Y. Sun, X. Shen et al., “Intracellular expression of Vitreoscilla hemoglobin improves production of Yarrowia lipolytica lipase LIP2 in a recombinant Pichia pastoris,” Enzyme and Microbial Technology, vol. 50, no. 1, pp. 22–28, 2012. View at Publisher · View at Google Scholar · View at Scopus
  20. M. Urgun-Demirtas, K. R. Pagilla, and B. Stark, “Enhanced kinetics of genetically engineered Burkholderia cepacia: role of vgb in the hypoxic cometabolism of 2-CBA,” Biotechnology and Bioengineering, vol. 87, no. 1, pp. 110–118, 2004. View at Publisher · View at Google Scholar · View at Scopus
  21. J.-M. Wu and W.-C. Fu, “Intracellular co-expression of Vitreoscilla hemoglobin enhances cell performance and β-galactosidase production in Pichia pastoris,” Journal of Bioscience and Bioengineering, vol. 113, no. 3, pp. 332–337, 2012. View at Publisher · View at Google Scholar · View at Scopus
  22. A.-S. Xiong, Q.-H. Yao, R.-H. Peng et al., “High level expression of a synthetic gene encoding Peniophora lycii phytase in methylotrophic yeast Pichia pastoris,” Applied Microbiology and Biotechnology, vol. 72, no. 5, pp. 1039–1047, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. J. K. Yang and L. Y. Liu, “Codon optimization through a two-step gene synthesis leads to a high-level expression of Aspergillus niger lip2 gene in Pichia pastoris,” Journal of Molecular Catalysis B: Enzymatic, vol. 63, no. 3-4, pp. 164–169, 2010. View at Publisher · View at Google Scholar · View at Scopus
  24. J.-M. Wu, S.-Y. Wang, and W.-C. Fu, “Lower temperature cultures enlarge the effects of Vitreoscilla hemoglobin expression on recombinant pichia pastoris,” International Journal of Molecular Sciences, vol. 13, no. 10, pp. 13212–13226, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. H. Huang, P. Yang, H. Luo et al., “High-level expression of a truncated 1,3-1,4-β-D-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation,” Applied Microbiology and Biotechnology, vol. 78, no. 1, pp. 95–103, 2008. View at Publisher · View at Google Scholar · View at Scopus
  26. R. Mora-Lugo, M. Madrigal, V. Yelemane, and M. Fernandez-Lahore, “Improved biomass and protein production in solid-state cultures of an Aspergillus sojae strain harboring the Vitreoscilla hemoglobin,” Applied Microbiology and Biotechnology, vol. 99, no. 22, pp. 9699–9708, 2015. View at Publisher · View at Google Scholar
  27. F. Li, S. Yang, L. Zhao, Q. Li, and J. Pei, “Synonymous condon usage bias and overexpression of a synthetic xynB gene from Aspergillus Niger NL-1 in Pichia pastoris,” BioResources, vol. 7, no. 2, pp. 2330–2343, 2012. View at Google Scholar · View at Scopus
  28. Y. Wang, Z. Wang, Q. Xu et al., “Lowering induction temperature for enhanced production of polygalacturonate lyase in recombinant Pichia pastoris,” Process Biochemistry, vol. 44, no. 9, pp. 949–954, 2009. View at Publisher · View at Google Scholar · View at Scopus