BioMed Research International / 2016 / Article / Tab 1

Research Article

Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

Table 1

Expression of recombinant human insulin in Pichia pastoris as evaluated by ELISA.

Commercial insulinTime (hrs)Recombinant proinsulin
Protein conc.
(µg/mL)
OD 495Mut+ cloneMut clone
OD 495Protein conc. (µg/mL) OD 495Protein conc. (µg/mL)

50.695 0.03100.447 0.0150.473 0.020
100.706 0.015240.523 0.01080.587 0.12011
150.776 0.015720.749 0.023200.0818 0.02126
200.798 0.070961.155 0.130311.205 0.10135
250.802 0.1101440.870 0.030231.015 0.06029
300.870 0.09

Mut: methanol utilization +ve clone.
Mut: methanol utilization slow phenotype.

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