Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2016 (2016), Article ID 3529561, 10 pages
http://dx.doi.org/10.1155/2016/3529561
Research Article

Potential Osteoinductive Effects of Calcitriol on the m-RNA of Mesenchymal Stem Cells Derived from Human Alveolar Periosteum

1Department of Periodontics, Chang Gung Memorial Hospital, Linkou, Taiwan
2Chang Gung University, Taoyuan, Taiwan
3School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
4California Northstate University, College of Medicine, Elk Grove, CA, USA
5Division of Clinical Toxicology, Department of Nephrology, Chang Gung Memorial Hospital, Linkou, Taiwan
6Kidney Research Center, Chang Gung Memorial Hospital, Linkou, Taiwan
7Center for Tissue Engineering, Chang Gung Memorial Hospital, Linkou, Taiwan
8Department of Periodontics, Chang Gung Memorial Hospital, Taoyuan, Taiwan

Received 24 June 2016; Revised 18 October 2016; Accepted 30 October 2016

Academic Editor: Kunikazu Tsuji

Copyright © 2016 Hsiang-Hsi Hong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study characterized alveolar periosteum-derived mesenchymal stem cells (P-MSCs) and examined the hypothesis that 1,25-(OH)2D3 (calcitriol) exerts osteoinductive effects on P-MSCs. The mRNA expressions of alkaline phosphatase (ALP), bone sialoprotein (BSP), core-binding factor alpha-1 (CBFA1), collagen-1 (Col-1), osteocalcin (OCN), and vitamin D3 receptor (VDR) were assessed after incubation with calcitriol for 2 weeks. Vitamin C as positive control (Vit. C-p) increased ALP and CBFA1 mRNA expression at both 1 and 2 weeks and increased BSP and Col-1 mRNA expression only at the first week. A concentration of 10−8 M calcitriol enhanced ALP, CBFA1, Col-1, and OCN mRNA expression at both weeks and BSP mRNA expression at the first week. Furthermore, 10−7 M calcitriol increased the mRNA expressions of all compounds at both weeks, except that of CBFA1 at the first week. 10−8 M calcitriol and Vit. C-p enhanced ALP activity at the second and third weeks. The results revealed that 10−9, 10−8, and 10−7 M calcitriol induced osteoinduction in alveolar P-MSCs by increasing ALP, CBFA1, Col-1, and OCN mRNA expression. A 10−7 M calcitriol yielded a higher mRNA expression than Vit. Cp on VDR and OCN mRNA expression at both weeks and on Col-1 mRNA at the second week.