Transferrin Impacts Bacillus thuringiensis Biofilm Levels

Figure 1

(a) B. thuringiensis strains form biofilms in iron restrictive medium. CTM was inoculated to an OD₆₀₀ of 0.02 in vinyl plates and incubated for 24 hours at 30°C. Microtiter plates were inverted to remove unattached cells. Samples were stained with crystal violet and washed with PBS. Biofilm biomass was detected by the addition of alcohol decolorizer, which was measured at A₅₄₀. Data are presented as the average, with error bars indicating the standard deviation. (b) B. thuringiensis strains grow in iron restrictive medium. CTM was inoculated to an OD₆₀₀ of 0.02 in vinyl plates and incubated for 24 hours at 30°C. Culture turbidity was measured at OD₆₂₀. Samples are presented as the average, with error bars indicating the standard deviation.

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