TNFα Affects Ciliary Beat Response to Increased Viscosity in Human Pediatric Airway Epithelium

Figure 3

TNFα incubation affects intracellular calcium levels to prevent the collapse of CBF in response to viscous loading. ((a) and (c)) Time course of the changes in CBF in ciliated cells after 48 hours of treatment with TNFα (10 ng/mL) or control solution (HBSS) exposed to 10% dextran in the presence or absence of intracellular (Thapsigargin 2.5 μM) (a) or extracellular calcium (Gadolinium 100 μM) (c) blockers. ((b) and (d)) Area under the curve of (a) (b) and (c) (d) between 5 and 30 min of treatment. Each bar represents the mean ± SEM for different treatments. significant differences (). corresponds to individual ciliated cell measure from three patient.

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