Modification of the Sweetness and Stability of Sweet-Tasting Protein Monellin by Gene Mutation and Protein Engineering

Figure 1

Nucleotide and amino acid sequence of single-chain monellin (MNEI) construct. The six histidines introduced at the N-terminal of the MNEI construct and the start codon are underlined, respectively. The thrombin cleavage site is denoted by an arrow. The stop codon of MNEI is annotated as asterisk. The E23A and C41A mutants sites with considerably improved thermostability or sweetness are boxed.