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BioMed Research International
Volume 2016, Article ID 3708187, 8 pages
http://dx.doi.org/10.1155/2016/3708187
Research Article

Ethylene-Induced Vinblastine Accumulation Is Related to Activated Expression of Downstream TIA Pathway Genes in Catharanthus roseus

1Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China
2College of Life Science, Northeast Forestry University, Harbin 150040, China

Received 13 December 2015; Revised 30 March 2016; Accepted 6 April 2016

Academic Editor: Sudhir Sopory

Copyright © 2016 Xi Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We selected different concentrations of ethephon, to stress C. roseus. We used qRT-PCR and HPLC followed by PCA to obtain comprehensive profiling of the vinblastine biosynthesis in response to ethephon. Based on our findings, the results showed that the high concentration of ethephon had a positive effect at both transcriptional and metabolite level. Meanwhile, there was a remarkable decrease of hydrogen peroxide content and a promoted peroxidase activity in leaves. The loading plot combination with correlation analysis suggested that CrPrx1 could be regarded as a positive regulator and interacts with ethylene response factor (ERF) to play a key role in vinblastine content and peroxidase (POD) activity. This study provides the foundation for a better understanding of the regulation and accumulation of vinblastine in response to ethephon.