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BioMed Research International
Volume 2016, Article ID 3758941, 7 pages
http://dx.doi.org/10.1155/2016/3758941
Research Article

High Efficient Expression, Purification, and Functional Characterization of Native Human Epidermal Growth Factor in Escherichia coli

1School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China
2Guangdong Province Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou 510006, China

Received 25 July 2016; Accepted 5 September 2016

Academic Editor: Jiazhang Lian

Copyright © 2016 Yi Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [8 citations]

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  • Anna Lena Gell, Nadja Groysbeck, Christian F. W. Becker, and Anne C. Conibear, “A comparative study of synthetic and semisynthetic approaches for ligating the epidermal growth factor to a bivalent scaffold,” Journal of Peptide Science, 2017. View at Publisher · View at Google Scholar
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  • Rabiei Farinaz, and Motovali-Bashi Majid, “High efficient prokaryotic expression and purification of bioactive human growth hormone using a cleavable self-aggregating tag,” Gene Reports, 2018. View at Publisher · View at Google Scholar
  • Thomas Baier, Dana Kros, Rebecca C. Feiner, Kyle J. Lauersen, Kristian M. Müller, and Olaf Kruse, “ Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii ,” ACS Synthetic Biology, 2018. View at Publisher · View at Google Scholar
  • Meng Wang, Jinglian Lin, Yi Ma, Shan Li, Jufang Wang, Kaiwen Zheng, Minhua Huang, and Xiaochun Luo, “Rapid and efficient production of cecropin A antibacterial peptide in Escherichia coli by fusion with a self-aggregating protein,” BMC Biotechnology, vol. 18, no. 1, 2018. View at Publisher · View at Google Scholar
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  • Han Luo, Lifu Hu, Buyong Ma, Meiqi Zhao, Manyu Luo, Qing Deng, Shaorong Deng, Hao Ye, Tong Lin, Junsheng Chen, Tao Wang, Jianwei Zhu, and Huili Lu, “Molecular dynamics based improvement of the solubilizing self-cleavable tag Zbasic-ΔI-CM application in the preparation of recombinant proteins in Escherichia coli,” Biochemical and Biophysical Research Communications, 2019. View at Publisher · View at Google Scholar
  • Rebecca C. Feiner, Ina Pennè, Benjamin Müller, and Kristian M. Müller, “ EGF-mCherry Fusion Protein Expressed in E. coli Shows Product Heterogeneity but a High Biological Activity ,” Biochemistry, 2019. View at Publisher · View at Google Scholar