Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Contribute to Chondrogenesis in Coculture with Chondrocytes

Figure 2

COL2 protein levels were characterized by immunofluorescence. Immunofluorescence staining of COL2 was shown in red. Nuclei were stained with DAPA (4′,6-diamidino-2-phenylindole, blue color). (a) hACs alone; (b) UCB-MSCs alone; (c) hUCB-MSCs cultured with growth factors; (d) direct coculture (1 : 1); (e) direct coculture (3 : 1); (f) direct coculture (5 : 1); (g) indirect coculture (UCB-MSCs); and (h) indirect coculture (hACs). Scale bar is 100 μm. .

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