Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Contribute to Chondrogenesis in Coculture with Chondrocytes

Figure 3

Gene expression was quantified with quantitative polymerase chain reaction. (A) SOX9, (B) Col1a1, and (C) Col2a1. Statistically significant differences were found by one-way ANOVA in all three genes (). (a) hACs alone; (b) UCB-MSCs alone; (c) hUCB-MSCs cultured with growth factors; (d) direct coculture (1 : 1); (e) direct coculture (3 : 1); (f) direct coculture (5 : 1); (g) indirect coculture (UCB-MSCs); and (h) indirect coculture (hACs).