Table 2: Differentially regulated proteins in biofilm samples treated with two disinfectants as determined using LC-LTQ/Orbitrap MS.

DescriptionPGAEthanol (E)Chloramine T (C)Differences between disinfectants
MedianQDMedianQD

AdherenceClumping factor AQ6GB45233.9900.119453.6500.648
Clumping factor BQ6G64462.3580.159110.8450.316EC
Elastin-binding protein EbpSQ6G98320.8790.16481.2590.440
EnolaseA7WZT2880.087831.1310.203
Extracellular adherence protein EapD9RNP143.2710.37962.8070.144
Fibrinogen-binding proteinP6879921.3340.10942.1140.059
Fibronectin-binding protein AQ6G6H340.07981.6550.125EC
Immunoglobulin-binding protein AQ8NYT0750.058450.010
Immunoglobulin-binding protein sbiQ6G6Q3471.3680.263381.0880.165EC
Iron-regulated surface determinant protein AA7X14851.3580.058130.040EC

Cell wall synthesis and organizationLipoteichoic acid synthaseQ2FIS2130.322150.4070.292
N-acetylmuramoyl-L-alanine amidase sle1Q2FJH790.8850.36140.008EC
Probable transglycosylase SceDA7X6T922.0330.02540.050EC

Physiological proteinsCold shock protein CspAQ2FH3670.0670.33540.009
Large-conductance mechanosensitive channelA7X20461.5270.06541.3060.029

Transport through membraneFoldase protein PrsAA7X3U8191.2870.342170.066EC

Uncharacterized proteinsUncharacterized lipoprotein MW0073Q8NYU011.2560.00070.8520.072
Uncharacterized lipoprotein SAS2259Q6G6V2161.4610.177151.2590.106
UPF0478 protein SA1560Q7A531220.102271.2450.125EC

Virulence factorsPenicillin-binding protein 1Q8NX3741.2470.04120.3230.009
Phospholipase CA5IUH145.9092.23125.2520.118
Secretory antigen SsaA-like proteinA6QEX425.5780.1060
Serine-aspartate repeat-containing protein CQ6GBS6322.8190.404120.054EC
Staphylococcal secretory antigen ssaA2Q2G2J2224.0882.288210.135EC
Virulence factor EsxAQ5HJ91162.0020.980172.2611.384

PGA: protein group accession number; : number of peptides; QD: quartile deviation.
Italic/bold numbers represent statistically significant fold changes in the indicated proteins (upregulated/downregulated) (; ; Wilcoxon signed rank test with theoretical median = 1.0).
Statistically significant differences between disinfectants (; ; Mann-Whitney test followed by post hoc tests).